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Schaffer collateral → CA1 temporal summation

stratum radiatum · frequency-dependent EPSP summation & short-term facilitation

Stimulus train

6
25 Hz
2 Hz100 Hz
Inter-spike interval: 40.0 ms. Summation only matters when this is short compared to the decay below.

Postsynaptic integration

20 ms
5 ms (fast)60 ms (slow)
Stands in for the postsynaptic membrane time constant. Longer τ lets each EPSP linger, so more of it is still there when the next one arrives.
If summed depolarization reaches −55 mV, a somatic action potential is triggered. Only shown in EPSP mode — field potentials here don't spike.

Presynaptic facilitation (residual Ca²⁺)

35%
How much leftover Ca²⁺ from prior pulses boosts release on the next one. Zero = every EPSP is the same size; only passive summation is left.
70 ms
20 ms200 ms

Presynaptic depression (vesicle depletion)

30%
Fraction of the currently-available vesicle pool released per spike (scaled by facilitation, so a more facilitated spike also depletes more). Zero = an inexhaustible pool, facilitation only.
400 ms
50 ms (fast)1500 ms (slow)
How quickly depleted resources refill between spikes. Facilitation and depression now compete for each EPSP's amplitude.

Run

Oscilloscope — 3 channel

presynaptic AP presynaptic [Ca²⁺] EPSP spike / threshold
t = 0 at first pulse (ms)

Session readout

Inter-spike interval
—
Train duration
—
Peak residual [Ca²⁺]
—
Peak postsynaptic response
—
STP ratio (last / 1st EPSP)
—
Min vesicle pool
—
Postsynaptic spike
—
Adjust the controls, then click Run Sweep to trigger the oscilloscope.
Laboratorio de Neurofisiología · USACH