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Patch-Clamp Lab — Single Channel Recording (Naᵥ-type, 3-state)

Single channel trace

One channel, one sweep. The gate traces on top show each gating particle moving between its resting (low) and activated (high) position, time-aligned with the current below.

Stateclosed
Openings2
First latency0.090 ms
Po (during pulse)0.0385
Closed cursor (I)0.000 pA
Open cursor (I)-1.125 pA
ΔI = I(open) − I(closed)-1.125 pA
Drag the two dashed cursor lines onto the trace — Closed onto the baseline, Open onto an open level — then press Record point to log the test voltage and the measured current step (ΔI) below.
Recorded measurements
#Test V (mV)ΔI (pA)
I–V plot

Multiple sweeps

The same channel, repeated trials at the same test potential — trial-to-trial variability in latency, opening count and duration. As on a real rig, one sweep is acquired every 500 ms and each new sweep is drawn below the previous one. Right-click any trace to drop a marker and log its (voltage, current) into the table.

The settings in the side panel are captured when a run starts; changes apply to the next run. The number of sweeps locks while a run is in progress — press Reset to change it.
Sweep 0 / 20
Per-sweep summary
SweepFirst latency (ms)# openingsTotal open time (ms)Po
Picked points
#SweepTime (ms)Voltage (mV)I (pA)
I–V plot

Ensemble average

Average many independent channels (or repeated sweeps) and the noisy single-channel flickers become the smooth macroscopic current students recognize from whole-cell recordings. Sweeps are acquired one every 500 ms and overlaid; when the last one arrives the average is computed and drawn on top. Right-click the bold average trace to log its (voltage, current) into the table.

The settings in the side panel are captured when a run starts; changes apply to the next run. N locks while a run is in progress — press Reset to change it.
Sweep 0 / 150
The bright trace is the sweep just recorded; earlier sweeps fade into the background. The bold line, drawn after the last sweep, is the average of all N sweeps — i × Po(t), which scaled by the number of channels in a cell is exactly what the whole-cell macroscopic current represents.
Picked points
#Time (ms)Voltage (mV)I (pA)
Macroscopic-like I–V plot

Dwell-time histograms

Runs 200 pulses one at a time, like watching an oscilloscope sweep — most informative for the Kᵥ channel, whose non-inactivating flicker gives many open/closed events per pulse. Every 5th pulse, the closed- and open-time histograms below update with all events collected so far (first latency and any dwell still in progress at pulse end are excluded, to avoid bias).

These fields lock once a run starts (including while paused) so the accumulated histogram always reflects one consistent protocol — press Reset to change them.
Pulse 0 / 200
Closed times
n=0
Open times
n=0
Laboratorio de Neurofisiología · USACH